Embryonic, Meiotic and Molecular Analysis of Apomictic Cassava (Manihot esculenta Crantz)
نویسنده
چکیده
Apomixis genes have been successfully transferred to cassava by hybridizing it with the wild species, M. glaziovii. An interspecific hybrid of cassava and M. glaziovii was left for open pollination during three subsequent generations. Seven sibs and their maternal progenitor of the fourth generation were genotyped using five microsatellite loci previously developed for cassava. All sibs were identical with each other and with their maternal progenitor. Sibs from M. glaziovii itself proved to be identical when examined by the same microsatellite loci. Cytogenetic analysis shows the apomictic clone has chromosome constitution of 2n=38. The embryonic study revealed formation of multiembryonic aposporic embryo sacs.
منابع مشابه
Cassava, Manihot esculenta Crantz, genetic resources: origin of the crop, its evolution and relationships with wild relatives.
About 98 species of Manihot are known. All of them are native to the New World and are concentrated in four regions in Brazil and Central America. All the Manihot species so far examined have 2n = 36 chromosomes. Interspecific hybrids between cassava and its wild relatives show relatively normal meiosis, and further generations can be obtained. Electrophoresis shows affinity among wild species ...
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